Oligonucleotides
We synthesize custom DNA and RNA oligonucleotides: PCR primers, hydrolysis probes for qPCR and oligos with chemical modifications. Synthesis scales from 40 nmol to 10 µmol and above; purification by desalting, HPLC or PAGE depending on the application.
Types and parameters
PCR primers
DNA oligonucleotides for PCR and Sanger sequencing, available at 40 nmol, 200 nmol and 1 µmol scale with desalting or HPLC purification.
Request a quote →Hydrolysis probes (qPCR / TaqMan)
Probes with a fluorophore and quencher (BHQ-1, BHQ-2, Iowa Black) for real-time quantitative PCR and molecular diagnostics.
Request a quote →5' and 3' modifications
Fluorophores (FAM, HEX, Cy3, Cy5, TAMRA), quenchers, biotin, aminolinker, phosphate and thiol groups at the 5' or 3' end.
Request a quote →Purification
Desalting (standard), HPLC purification (modified oligos, probes) or PAGE (oligos >40 nt, clinical applications).
Request a quote →Workflow
Specification
You send the sequence, synthesis scale, modifications and required purification method.
Synthesis
Phosphoramidite synthesis with coupling yield control at every cycle.
Purification and analysis
Purification by the selected method (desalting / HPLC / PAGE) and purity verification.
Delivery
Shipped as a lyophilizate or in solution (TE or water) with a product sheet and MS spectrum (for probes and modified oligos).
Who is it for?
Oligonucleotide synthesis quote
Send the sequence, synthesis scale, modifications and purification method. We will reply with a quote.